Western Blotting

1. Tris-buffered saline (TBS): 137 mM NaCl, 5 mM KCl, 1.4 mM Na2HPO4, 25 mM

2. Solubilization buffer: 1% sodium dodecyl sulfate (SDS), 20 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA.

4. Fluorescamine solution: 0.2 mg fluorescamine dissolved in 1 mL acetonitrile.

5. Transfer buffer: 25 mMTris, 193 mM glycine, pH 8.5.

7. Blocking buffer: 7% milk in TBS-0.3% Tween-20

8. Secondary antibody dilution buffer: 10 mL blocking buffer plus 20 mL TBS-0.1% Tween-20

0 0

Post a comment