Methods

The methods described in this section were developed for 3T3-L1 adipocytes, a murine-derived tissue culture model system for adipose tissue (see Chapter 9). However, the protocols should be easily adapted for use with other cell lines or tissues expressing insulin receptor and insulin receptor substrates. However, it should be noted that IR and IRS proteins are expressed in many cell types at levels considerably lower than in 3T3-L1 adipocytes. The volumes and quantities detailed here are appropriate for adipocytes cultured in 6-well dishes (approx 150,000 cells/well; see Note 2).

Diabetes 2

Diabetes 2

Diabetes is a disease that affects the way your body uses food. Normally, your body converts sugars, starches and other foods into a form of sugar called glucose. Your body uses glucose for fuel. The cells receive the glucose through the bloodstream. They then use insulin a hormone made by the pancreas to absorb the glucose, convert it into energy, and either use it or store it for later use. Learn more...

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