Materials Reagents

2.1.1. Glycogen Synthase Assay

1. Glycogen synthase buffer (GSB): 50 mMHEPES (pH 7.8), 10 mMEDTA, and 100 mM NaF.

2. Krebs Ringer buffer with 30 mMHEPES (pH 7.4) (KRBH): 136 mMNaCl, 30 mM HEPES (pH 7.4), 5 mM sodium phosphate (pH 7.4), 4.7 mM KCl, 1 mM MgSO4, 1 mM CaCl.

5. Glucose-6-phosphate (Sigma cat. no. G7879).

6. Rat liver glycogen, type III (Sigma cat. no. G8876).

7. 70% Ethanol stocks, 4°C and room temperature (RT).

8. Whatman GF/A filters, 24 mm in diameter (cat. no. 1820-024).

2.1.2. Glycogen Synthesis Assay

1. Krebs Ringer buffer with 30 mM HEPES (pH 7.4) (KRBH).

2. 14C-Glucose (250 mCi/mmol).

3. Rat liver glycogen, type III (Sigma cat. no. G8876).

4. Potassium hydroxide pellets.

5. 95% Ethanol, room temperature.

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