Deoxyglucose Uptake Assay

1. Blastocysts are moved by mouth pipet to HTF media supplemented with D-glucose to give a final concentration of 5.6 mM and containing 500 nM insulin for 30 min. Following this preincubation, they are moved in groups of 10-20 into Ringers wash and then into media containing 200 iM 2-DG for 15 min. A basal group is placed in HTF with 5.6 mM D-glucose with no insulin added.

2. The embryos are then removed, washed in DG-free, BSA-free Ringers solution for 1 min, and then transferred with 0.5-1 ^L of the same salt solution onto a glass slide with a braking pipet. The embryo is then quick-frozen immediately by dipping the glass slide into isopentane brought to its freezing point (— 170°C) with liquid N2.

3. The specimens are then freeze-dried on the slide at — 35°C in a glass vacuum tube at a vapor pressure of <0.01 mm Hg. The slides are then stored at —20°C under reduced pressure.

Supplements For Diabetics

Supplements For Diabetics

All you need is a proper diet of fresh fruits and vegetables and get plenty of exercise and you'll be fine. Ever heard those words from your doctor? If that's all heshe recommends then you're missing out an important ingredient for health that he's not telling you. Fact is that you can adhere to the strictest diet, watch everything you eat and get the exercise of amarathon runner and still come down with diabetic complications. Diet, exercise and standard drug treatments simply aren't enough to help keep your diabetes under control.

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